Right, I understand all of that. My question is only from Effect Send -> 25ft of cable -> Buffered pedals. I'm not worried about the return because at least one pedal in the loop is buffered. If the Effect Send is low impedance already, then I wouldn't need a buffer. If it's high impedance, then I would.
I don't have any 25ft cables to try so I removed the buffer from my board and ran my entire board in the DT-25's effects loop with all of the pedals off. There was a significant loss of high end so I'm thinking a buffer is more than likely going to be required :(, although, it's not a perfect test and the loss may be less with a single cable than a bunch of smaller patches and TB pedals.